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Which condition affects the accuracy of spectrophotometry when measuring absorbance?

A)Stray light within the beam
B)Variations in refractive index
C)Detector temperature fluctuations occur
D)Imperfect alignment of cuvettes

💡 Explanation

Stray light artificially inflates the measured transmittance; therefore, it lowers the apparent absorbance because the detector receives light bypassing the sample. This systematic error skews results, rather than refractive index that affects scattering.

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