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Which consequence occurs when an enzyme unfolds completely?

A)Active site loses function
B)Substrate binding strengthens
C)Reaction rate increases sharply
D)Enzyme becomes more thermostable

💡 Explanation

The active site loses function because unfolding disrupts the protein's tertiary structure via denaturation, a change in the "function" form affecting the enzyme's catalytic ability therefore substrate affinity decreases rather than strengthens under stress, and activity diminishes.

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