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Which consequence results when poor spectral overlap exists in Förster resonance energy transfer (FRET)?

A)Increased fluorophore photobleaching occurs
B)Reduced energy transfer efficiency occurs
C)Increased donor fluorescence lifetime
D)Enhanced acceptor emission strength

💡 Explanation

Reduced energy transfer efficiency occurs because the transfer rate is proportional to spectral overlap through dipole-dipole coupling; this is the FRET Mechanism. Therefore efficient energy transfer is decreased, rather than increased photobleaching due to excited-state relaxation occurring.

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