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← ScienceWhich consequence results when poor spectral overlap exists in Förster resonance energy transfer (FRET)?
A)Increased fluorophore photobleaching occurs
B)Reduced energy transfer efficiency occurs✓
C)Increased donor fluorescence lifetime
D)Enhanced acceptor emission strength
💡 Explanation
Reduced energy transfer efficiency occurs because the transfer rate is proportional to spectral overlap through dipole-dipole coupling; this is the FRET Mechanism. Therefore efficient energy transfer is decreased, rather than increased photobleaching due to excited-state relaxation occurring.
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