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← ScienceWhich effect diminishes structured illumination microscopy resolution when imaging thick specimens?
A)Loss of illumination coherence length✓
B)Increased sample refractive index mismatch
C)Chromatic aberration from lens dispersion
D)Reduced fluorophore excitation cross-section
💡 Explanation
Structured illumination relies on high coherence to generate interference patterns; scattering introduces path length variations because it exceeds the coherence length, therefore fringes blur, rather than refractive mismatches alone, reducing resolution; cross-section/chromaticity are secondary.
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