Which mechanism causes quenching of fluorescence when fluorophore's excited state orbital interacts with nearby molecule's orbital?

💡 Explanation

When fluorophore excited state interacts with neighbor, Förster resonance energy transfer (FRET) occurs because quantum mechanical dipole-dipole coupling transfers energy non-radiatively rather than radiative fluorescence decay. Therefore, non-radiative energy transfer causes quenching, rather than others, which involve different decay mechanisms.

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