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← ScienceWhich mechanism enhances resolution in stimulated emission depletion microscopy?
A)Spatial modulation of fluorescence.✓
B)Temporal gating with fast detectors.
C)Two-photon excitation reduction.
D)Wave interference pattern collapse.
💡 Explanation
Spatial modulation of fluoresence enhances resolution because stimulated emission selectively suppresses fluorescence at the edges using a depletion beam. This narrowing sharpens the effective point spread function therefore improving resolution, rather than alternative methods which do not directly shape the signal.
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