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← ScienceWhich mechanism governs reaction rate increase when enzyme concentration significantly exceeds substrate concentration in biological assay?
A)Active site saturation by inhibitor molecules
B)Enzyme denaturation by product accumulation
C)Diffusion limitation of substrate binding✓
D)Reversible covalent modification by cofactors
💡 Explanation
When enzyme concentration exceeds substrate, the reaction rate becomes limited by diffusion because the substrate molecules struggle to access vacant enzyme active sites due to Brownian motion, rather than actual catalytic turnover. Therefore diffusion limitation becomes dominant, rather than saturation, denaturation, or modification which require different conditions.
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