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Which mechanism limits butanol production rate in bioreactors?

A)Enzyme catalyst active site saturation
B)Substrate limitation mass transport effects
C)Carbon dioxide solubility limit reached
D)Nutrient depletion in vessel center

💡 Explanation

Enzyme active site saturation limits alcohol production by competitive inhibition, because the enzyme-substrate complex formation plateaus. Therefore overall, butanol production reaches a maximum, rather than continuing to increase linearly with substrate level, due to constant active site limit.

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