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Which outcome occurs when an inhibited enzyme loses cofactor binding in a metabolic pathway?

A)Decreased flux through pathway results
B)Increased feedback inhibition of enzyme
C)Pathway activity shifts towards catabolism
D)Non-competitive inhibitor becomes competitive

💡 Explanation

Loss of cofactor binding eliminates the enzyme's activity due to disruption of the *active site*, therefore decreasing the rate of reaction it catalyzes and causing lower metabolic flux, rather than the other outcomes seen given sufficient binding affinity because cofactor dictates active enzyme status.

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