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Which outcome reduces the reaction rate when increasing temperatures for enzyme-catalyzed reactions?

A)Enzyme active site denaturation prevails
B)Substrate collision frequency greatly increases
C)Enzyme-substrate affinity constantly increases
D)Product inhibition becomes less significant

💡 Explanation

Enzyme active site denaturation reduces the reaction rate because increased temperatures cause unfolding and loss of the active site's structure, therefore substrate binding decreases, rather than reaction rates increasing due to more substrate collisions.

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