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Which risk increases when a pulsed laser's power increases in confocal microscopy?

A)Sample photobleaching becomes more prominent
B)Spherical aberration severely impacts resolution
C)Raman scattering overwhelms fluorescence signal
D)Laser speckle obscures detailed imaging

💡 Explanation

Sample photobleaching is more pronounced because higher laser power accelerates fluorophore decomposition through photolysis, therefore the fluorescence signal degrades rapidly, rather than the other listed optical artifacts affecting primary imaging quality.

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