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Which risk increases when the laser power drifts upward in differential interference contrast (DIC) microscopy?

A)Reduced signal-to-noise ratio
B)Increased photomultiplier tube burnout
C)Specimen photodamage accelerates
D)Resolution beyond Abbe limit

💡 Explanation

Increased optical power induces specimen photodamage through photochemical reactions; this mechanism accelerates cell stress. Photodamage increases because of direct light energy absorption; therefore the sample degrades, rather than improving resolution or signal which require minimizing photodamage.

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