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← ScienceWhich kinetic outcome affects an enzyme-substrate reaction increasing temperatures?
A)Enzyme denaturation overcomes reaction velocity✓
B)Substrate depletion limits reaction rate
C)V-Max increases despite enzyme activity
D)Michaelis constant (Km) stays stable
💡 Explanation
Enzyme denaturation occurs because increased temperatures disrupt an enzyme's three-dimensional structure via unfolding process; therefore, catalytic activity ceases above a certain threshold, rather than Michaelis constant (Km) remaining stable or just substrate depletion being the only parameter.
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