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Which kinetic outcome affects an enzyme-substrate reaction increasing temperatures?

A)Enzyme denaturation overcomes reaction velocity
B)Substrate depletion limits reaction rate
C)V-Max increases despite enzyme activity
D)Michaelis constant (Km) stays stable

💡 Explanation

Enzyme denaturation occurs because increased temperatures disrupt an enzyme's three-dimensional structure via unfolding process; therefore, catalytic activity ceases above a certain threshold, rather than Michaelis constant (Km) remaining stable or just substrate depletion being the only parameter.

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