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Which mechanism limits achievable resolution in optical microscopy due to molecular properties?

A)Vibrational mode absorption broadening
B)Quantum tunneling in the objective lens
C)Refractive index fluctuations of immersion oil
D)Chromatic aberration in the light source

💡 Explanation

When optical microscopy attempts to resolve molecules, vibrational mode absorption Broadening occurs because molecules absorb light at specific vibrational frequencies, causing blurring and reduced resolution, affecting the imaging precision. Therefore vibrational mode absorption matters, rather than tunneling, aberration, or refractive, which have different root cause and process.

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