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Which outcome occurs when an enzyme reaches saturation?

A)Reaction rate plateaus at Vmax
B)Inhibitors irreversibly deactivate the protein
C)Enzyme active sites diminish exponentially
D)The Michaelis constant (Km) vanishes

💡 Explanation

When substrate concentration sufficiently increases, all enzyme active sites get occupied; therefore, the reaction rate reaches its maximum value (Vmax) as substrate concentration increases utilizing zero-order kinetics, rather than unlimited progression because *enzyme saturation* limits throughput.

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