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Which outcome occurs when excessive non-competitive inhibitor binds an enzyme?

A)Reaction rate experiences a Vmax decrease
B)Reaction achieves thermodynamically unstable equilibrium
C)Substrate affinity displays an apparent increase
D)Inhibition reverses upon substrate addition

💡 Explanation

Enzyme activity decreases because *conformational change* reduces its catalytic efficiency regardless of substrate concentration, thereby decreasing the Vmax; therefore the rate decreases, rather than changing substrate affinity which occurs in competitive inhibition.

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